Protein digestion is another method in which the complex molecules of the proteins are digested with the help of the special type of the enzymes such as trypsin, chymotrypsin and pepsins. Nonetheless, the process of DNA digestion is actually the process of vertical cutting, contrary to the denaturation process of the PCR. The polymerase chain reaction is the best example of the method for horizontal cutting while restricting digestion is the best example of vertical cutting. “The helicase cleaves the DNA horizontally by breaking the hydrogen bond between two strands during the replication while the endonuclease cut DNA vertically by breaking the phosphodiester bond.” In the vertical cutting, the phosphodiester bonds between the adjacent nucleotides are broken whereas in horizontal cutting the hydrogen bonds between the nucleotides of the opposite strands are broken. Nucleases such as endonucleases and exonuclease are the enzyme used to cut the DNA, however, it cuts DNA vertically. Whereas, Hydroxyurea, camptothecin are chemicals that cut DNA horizontally. UV radiation, gamma rays, Heat and centrifugation are several physical methods which cut DNA vertically as well as horizontally. The process of the DNA digestion creates mutation into the Genome. The process of cutting or cleaving DNA into smaller fragments with the help of the chemicals, physical and enzymatic methods are called the process of the DNA digestion. Factors affecting the restriction digestion:.In vitro process of Restriction digestion.different techniques of genetic engineering such as gene editing, CRISPR-CAS9, genetic manipulation, DNA sequencing and DNA microarray was simultaneously evolved as we. Īround the era of the 1980s, the idea of cutting DNA with the help of the specific type of endonuclease was more clear and due to this the era of the genetic engineering was evolve. Interestingly, the phenomenon of endonuclease in bacteria was first explained in the 1950s by Salvador Luria and Giuseppe Bertani. “Endonucleases are DNA manipulating tool, a molecular scissor found in wide varieties of prokaryotes, especially, in bacteria.” Arber were awarded Nobel prize in 1978 for discovering the endonuclease. However, the term was first explained by W. The first restriction endonuclease was discovered into the year 1970 (HindII) by Smith, Thomas Kelly and Kent Wilcox. Restriction endonucleases cut or cleave DNA at a specific location at their specific recognition site. Restriction digestion results analysis.The factors affecting restriction digestion.The in vitro process of restriction digestion.Also on how it is applicable in the disease and how we can analyse the results of the restriction digestion.įurthermore, we will interpret and analyse the results of the restriction digestion. The core value of the present article is on the process of the restriction digestion and its role in the detection of mutation. The process of cleaving DNA at a particular location with the help of the specific type of restriction endonuclease enzyme which helps in mapping, polymorphism study and studying mutation called as restriction digestion.įor understanding the process of the restriction digestion we have to first understand several terminologies such as what is digestion, what is DNA digestion, what is restriction endonuclease and what are the nucleases.
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